STATE_TRANSITION_LEFT__DIF_dimer_CACT_SLMB-RIGHT__DIF_dimer_phosphoCACT_UBI_SLMB-_re42
type: BiochemicalReaction
conversion direction: LEFT_TO_RIGHT
xref: STATE TRANSITION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re42 187
participant stoichiometry: PARTICIPANT STOICHIOMETRY re42 188
left: DIF dimer/CACT SLMB
interaction type: STATE_TRANSITION
right: DIF dimer phosphoCACT UBI SLMB

SLMB
type: Protein
entity reference: SLMB
feature: COMPLEX PARTICIPANT BINDING FEATURE s160 sa101 144
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

HOMODIMER ID 10STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: TL
stoichiometric coefficient: 2.0

HOMODIMER ID 35STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DIF
stoichiometric coefficient: 2.0

CACT s104 sa73 rs1
type: ModificationFeature
modification type: phosphorylated residue
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_NAME=
comment: MODIFICATION_RESIDUE_ANGLE=3.141592653589793

TL
type: Protein
entity reference: TL
feature: ACTIVE 9

nucleus
type: CellularLocationVocabulary
xref: GO GO:0005634 2

SPZ
type: Protein
entity reference: SPZ

SPZ
type: Protein
entity reference: SPZ

P06339
type: UnificationXref
id: P06339
db: PANTHER Pathway Component

P06338
type: UnificationXref
id: P06338
db: PANTHER Pathway Component

uniprot P08953 4
type: UnificationXref
id: P08953
db: uniprot

P06337
type: UnificationXref
id: P06337
db: PANTHER Pathway Component

EA
type: Protein
entity reference: EA
feature: ACTIVE 9
comment: SPECIES_TYPE=PROTEIN

HOMODIMER ID 41STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SPZ
stoichiometric coefficient: 2.0

PTHR24256:SF449
type: RelationshipXref
id: PTHR24256:SF449
db: PANTHER Family
relationship type: By Homology

TRANSPORT_LEFT__DL-RIGHT__DL-_re39
type: TransportWithBiochemicalReaction
conversion direction: LEFT_TO_RIGHT
xref: TRANSPORT
participant stoichiometry: PARTICIPANT STOICHIOMETRY re39 212
participant stoichiometry: PARTICIPANT STOICHIOMETRY re39 211
left: DL
interaction type: TRANSPORT
right: DL

DIF s168 sa107 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DIF
stoichiometric coefficient: 2.0

CACT s104 sa73 rs2
type: ModificationFeature
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_ANGLE=4.995363318667594
comment: MODIFICATION_RESIDUE_NAME=

DIF
type: Protein

target transcripts
type: RnaRegion
cellular location: nucleus
comment: SPECIES_TYPE=RNA

TUB
type: Protein
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

P06340
type: UnificationXref
id: P06340
db: PANTHER Pathway Component

P06342
type: UnificationXref
id: P06342
db: PANTHER Pathway Component

P06341
type: UnificationXref
id: P06341
db: PANTHER Pathway Component

HOMODIMER ID 43STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SPZ
stoichiometric coefficient: 2.0

P06348
type: UnificationXref
id: P06348
db: PANTHER Pathway Component

P06347
type: UnificationXref
id: P06347
db: PANTHER Pathway Component

CACT s96 sa63 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: CACT
stoichiometric coefficient: 1.0

CACT s167 sa106 rs1
type: ModificationFeature
modification type: phosphorylated residue
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_NAME=
comment: MODIFICATION_RESIDUE_ANGLE=3.141592653589793

P06349
type: UnificationXref
id: P06349
db: PANTHER Pathway Component

P06344
type: UnificationXref
id: P06344
db: PANTHER Pathway Component

P06346
type: UnificationXref
id: P06346
db: PANTHER Pathway Component

SPZ s58 sa40 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SPZ
stoichiometric coefficient: 2.0

HOMODIMER ID 32STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DIF
stoichiometric coefficient: 2.0

Drosophila melanogaster
type: BioSource
xref: ncbitaxon 7227 5

DL
type: Protein
entity reference: DL

DIF
type: Protein

DL s112 sa76 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DL
stoichiometric coefficient: 2.0

?
type: Protein
comment: SPECIES_TYPE=PROTEIN

CACT
type: Protein
entity reference: CACT
feature: CACT s167 sa106 rs2
feature: COMPLEX PARTICIPANT BINDING FEATURE s167 sa106 74
feature: CACT s167 sa106 rs1
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

TRANSPORT_LEFT__DIF-RIGHT__DIF-_re45
type: TransportWithBiochemicalReaction
conversion direction: LEFT_TO_RIGHT
xref: TRANSPORT
participant stoichiometry: PARTICIPANT STOICHIOMETRY re45 176
participant stoichiometry: PARTICIPANT STOICHIOMETRY re45 177
left: DIF
interaction type: TRANSPORT
right: DIF

DL dimer phosphoCACT
type: Complex
component stoichiometry: CACT s96 sa63 STOICHIOMETRY ID COMPLEX
component stoichiometry: HOMODIMER ID 25STOICHIOMETRY ID COMPLEX
component: DL
component: CACT
feature: COMPLEX BINDING FEATURE s148 csa13 129
feature: COMPLEX BINDING FEATURE s148 csa13 131
cellular location: cytosol

CACT s114 sa78 rs2
type: ModificationFeature
modification type: MODIFICATION FEATURE ubiquitinated
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_ANGLE=4.995363318667594
comment: MODIFICATION_RESIDUE_NAME=

ncbitaxon 7227 5
type: UnificationXref
id: 7227
db: ncbitaxon

HETERODIMER_ASSOCIATION_LEFT__DIF_dimer_phospho_CACT-_SLMB-RIGHT__DIF_dimer_CACT_SLMB-_re41
type: ComplexAssembly
spontaneous: true
conversion direction: LEFT_TO_RIGHT
xref: HETERODIMER ASSOCIATION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re41 190
participant stoichiometry: PARTICIPANT STOICHIOMETRY re41 191
participant stoichiometry: PARTICIPANT STOICHIOMETRY re41 189
left: SLMB
left: DIF dimer phospho CACT
interaction type: HETERODIMER_ASSOCIATION
right: DIF dimer/CACT SLMB

By Homology
type: RelationshipTypeVocabulary

DIF dimer phospho CACT
type: Complex
component stoichiometry: HOMODIMER ID 32STOICHIOMETRY ID COMPLEX
component stoichiometry: CACT s154 sa97 STOICHIOMETRY ID COMPLEX
component: DIF
component: CACT
feature: COMPLEX BINDING FEATURE s156 csa20 89
feature: COMPLEX BINDING FEATURE s156 csa20 87
cellular location: cytosol

CACT s167 sa106 rs2
type: ModificationFeature
modification type: MODIFICATION FEATURE ubiquitinated
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_ANGLE=4.995363318667594
comment: MODIFICATION_RESIDUE_NAME=

PLL
type: Protein
entity reference: PLL
feature: ACTIVE 9
feature: COMPLEX PARTICIPANT BINDING FEATURE s78 sa54 128
feature: PLL s78 sa54 rs1
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

TL s142 sa24 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: TL
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re42 188
type: Stoichiometry
physical entity: DIF dimer phosphoCACT UBI SLMB
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re42 187
type: Stoichiometry
physical entity: DIF dimer/CACT SLMB
stoichiometric coefficient: 1.0

PLL
type: ProteinReference
xref: P06338
standard name: pelle
comment: ENTITY_REFERENCE_PROTEIN_TYPE=GENERIC
comment: ENTITY_REFERENCE_NOTES= pelle Uniprot: Q05652
comment: ENTITY_REFERENCE_ID_DESC=Toll_pathway_drosophila.PROTEIN.GENERIC.PLL
member entity reference: Serine/threonine-protein kinase pelle

CATALYSIS
type: Catalysis
controlled: STATE_TRANSITION_LEFT__DIF_dimer_CACT_SLMB-RIGHT__DIF_dimer_phosphoCACT_UBI_SLMB-_re42
controller: SLMB

TUB
type: Protein
feature: COMPLEX PARTICIPANT BINDING FEATURE s39 sa31 48
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

CACT s114 sa78 rs1
type: ModificationFeature
modification type: phosphorylated residue
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_NAME=
comment: MODIFICATION_RESIDUE_ANGLE=3.141592653589793

SLMB s113 sa77 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SLMB
stoichiometric coefficient: 1.0

LD20892p
type: ProteinReference
xref: uniprot Q7K105 36
organism: Drosophila melanogaster

UNKNOWN_TRANSITION_LEFT__PLL-RIGHT__unknown_protein-_re27
type: Conversion
conversion direction: LEFT_TO_RIGHT
xref: UNKNOWN TRANSITION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re27 200
participant stoichiometry: PARTICIPANT STOICHIOMETRY re27 199
left: PLL
interaction type: UNKNOWN_TRANSITION
right: unknown protein

DIF
type: Protein

DL
type: ProteinReference
xref: P06344
standard name: dorsal
comment: ENTITY_REFERENCE_PROTEIN_TYPE=GENERIC
comment: ENTITY_REFERENCE_NOTES= embryonic polarity protein dorsal Uniprot: P15330
comment: ENTITY_REFERENCE_ID_DESC=Toll_pathway_drosophila.PROTEIN.GENERIC.DL
member entity reference: Embryonic polarity protein dorsal

TL
type: Protein
entity reference: TL
feature: COMPLEX PARTICIPANT BINDING FEATURE s142 sa24 96
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

NF-kappa-B inhibitor cactus
type: ProteinReference
xref: uniprot Q03017 20
xref: PTHR46680:SF1
organism: Drosophila melanogaster

SPZ dimer/ TL dimer/ MyD88/TUB
type: Complex
component stoichiometry: MyD88 s138 sa34 STOICHIOMETRY ID COMPLEX
component stoichiometry: HOMODIMER ID 8STOICHIOMETRY ID COMPLEX
component stoichiometry: TUB s137 sa33 STOICHIOMETRY ID COMPLEX
component stoichiometry: HOMODIMER ID 45STOICHIOMETRY ID COMPLEX
component: SPZ
component: TL
component: TUB
component: MyD88
feature: COMPLEX BINDING FEATURE s136 csa6 55
feature: COMPLEX BINDING FEATURE s136 csa6 53
feature: COMPLEX BINDING FEATURE s136 csa6 57
feature: COMPLEX BINDING FEATURE s136 csa6 51
cellular location: cytosol

PARTICIPANT STOICHIOMETRY re38 152
type: Stoichiometry
physical entity: DL
stoichiometric coefficient: 2.0

PARTICIPANT STOICHIOMETRY re38 153
type: Stoichiometry
physical entity: CACT
stoichiometric coefficient: 1.0

TL
type: ProteinReference
xref: P06337
standard name: Toll
comment: ENTITY_REFERENCE_NOTES=
comment: ENTITY_REFERENCE_ID_DESC=Toll_pathway_drosophila.PROTEIN.RECEPTOR.TL
comment: ENTITY_REFERENCE_PROTEIN_TYPE=RECEPTOR
member entity reference: Protein toll

PARTICIPANT STOICHIOMETRY re38 151
type: Stoichiometry
physical entity: DL dimer phosphoCACT UBI
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re18 167
type: Stoichiometry
physical entity: SPZ dimer/ TL dimer/ MyD88/TUB
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re18 165
type: Stoichiometry
physical entity: MyD88/TUB
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re18 166
type: Stoichiometry
physical entity: SPZ dimer/ TL dimer
stoichiometric coefficient: 1.0

PTHR44156:SF6
type: RelationshipXref
id: PTHR44156:SF6
db: PANTHER Family
relationship type: By Homology

ACTIVE 9
type: EntityFeature
comment: Active state

SLMB s109 sa74 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SLMB
stoichiometric coefficient: 1.0

PLL s53 sa39 rs1
type: ModificationFeature
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_NAME=
comment: MODIFICATION_RESIDUE_ANGLE=3.141592653589793

CATALYSIS
type: Catalysis
controlled: STATE_TRANSITION_LEFT__DIF_dimer_CACT-RIGHT__DIF_dimer_phospho_CACT-_re40
controller: unknown protein

TL
type: Protein
entity reference: TL
feature: ACTIVE 9

MyD88 s138 sa56 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: MyD88
stoichiometric coefficient: 1.0

CACT s154 sa97 rs2
type: ModificationFeature
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_ANGLE=4.995363318667594
comment: MODIFICATION_RESIDUE_NAME=

CACT s151 sa96 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: CACT
stoichiometric coefficient: 1.0

CACT
type: Protein
entity reference: CACT
feature: COMPLEX PARTICIPANT BINDING FEATURE s158 sa100 142
feature: CACT s158 sa100 rs1
feature: CACT s158 sa100 rs2
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

PARTICIPANT STOICHIOMETRY re23 205
type: Stoichiometry
physical entity: ?
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re23 204
type: Stoichiometry
physical entity: SPZ
stoichiometric coefficient: 2.0

PARTICIPANT STOICHIOMETRY re23 203
type: Stoichiometry
physical entity: SPZ full length
stoichiometric coefficient: 2.0

CATALYSIS
type: Catalysis
controlled: STATE_TRANSITION_LEFT__SPZ_dimer__TL_dimer__MyD88_TUB__PLL-RIGHT__SPZ_dimer__TL_dimer__MyD88_TUB__PLL-_re21
controller: PLL

DL
type: Protein
entity reference: DL

SPZ s51 sa38 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SPZ
stoichiometric coefficient: 2.0

CACT s90 sa60 rs2
type: ModificationFeature
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_ANGLE=4.995363318667594
comment: MODIFICATION_RESIDUE_NAME=

PARTICIPANT STOICHIOMETRY re43 182
type: Stoichiometry
physical entity: DIF dimer phospho UBI CACT
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re16 172
type: Stoichiometry
physical entity: MyD88/TUB
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re43 181
type: Stoichiometry
physical entity: DIF dimer phosphoCACT UBI SLMB
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re43 183
type: Stoichiometry
physical entity: SLMB
stoichiometric coefficient: 1.0

SLMB
type: Protein
entity reference: SLMB
feature: COMPLEX PARTICIPANT BINDING FEATURE s109 sa74 150
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

TUB
type: Protein
feature: ACTIVE 9
feature: COMPLEX PARTICIPANT BINDING FEATURE s137 sa55 60
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

PARTICIPANT STOICHIOMETRY re16 170
type: Stoichiometry
physical entity: MyD88
stoichiometric coefficient: 1.0

CACT s154 sa97 rs1
type: ModificationFeature
modification type: phosphorylated residue
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_NAME=
comment: MODIFICATION_RESIDUE_ANGLE=3.141592653589793

PARTICIPANT STOICHIOMETRY re16 171
type: Stoichiometry
physical entity: TUB
stoichiometric coefficient: 1.0

MyD88
type: Protein
entity reference: MyD88
feature: COMPLEX PARTICIPANT BINDING FEATURE s77 sa53 126
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

DL s91 sa61 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DL
stoichiometric coefficient: 2.0

PARTICIPANT STOICHIOMETRY re44 180
type: Stoichiometry
physical entity: CACT
stoichiometric coefficient: 1.0

CACT s90 sa60 rs1
type: ModificationFeature
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_NAME=
comment: MODIFICATION_RESIDUE_ANGLE=3.141592653589793

PANTHER Pathway P06217 1
type: UnificationXref
id: P06217
db: PANTHER Pathway

HOMODIMER ID 12STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: TL
stoichiometric coefficient: 2.0

SPZ dimer/ TL dimer
type: Complex
component stoichiometry: HOMODIMER ID 11STOICHIOMETRY ID COMPLEX
component stoichiometry: HOMODIMER ID 42STOICHIOMETRY ID COMPLEX
component: TL
component: SPZ
feature: COMPLEX BINDING FEATURE s49 csa7 81
feature: COMPLEX BINDING FEATURE s49 csa7 79
cellular location: cytosol

uniprot Q7K105 36
type: UnificationXref
id: Q7K105
db: uniprot

SPN27A
type: Protein
entity reference: SPN27A
feature: COMPLEX PARTICIPANT BINDING FEATURE s174 sa110 106
comment: SPECIES_TYPE=PROTEIN

TRUNCATION
type: RelationshipTypeVocabulary

STATE_TRANSITION_LEFT__DL_dimer_CACT-RIGHT__DL_dimer_phosphoCACT-_re25
type: BiochemicalReaction
conversion direction: LEFT_TO_RIGHT
xref: STATE TRANSITION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re25 201
participant stoichiometry: PARTICIPANT STOICHIOMETRY re25 202
left: DL_dimer CACT
interaction type: STATE_TRANSITION
right: DL dimer phosphoCACT

PLL s59 sa44 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: PLL
stoichiometric coefficient: 1.0

uniprot P48607 37
type: UnificationXref
id: P48607
db: uniprot

HOMODIMER ID 46STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SPZ
stoichiometric coefficient: 2.0

PARTICIPANT STOICHIOMETRY re44 178
type: Stoichiometry
physical entity: DIF dimer phospho UBI CACT
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re44 179
type: Stoichiometry
physical entity: DIF
stoichiometric coefficient: 2.0

Protein toll
type: ProteinReference
xref: uniprot P08953 4
xref: PTHR24365:SF537
organism: Drosophila melanogaster

EA s20 sa19 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: EA
stoichiometric coefficient: 1.0

uniprot Q9VCJ8 17
type: UnificationXref
id: Q9VCJ8
db: uniprot

Spaetzle-processing enzyme
type: ProteinReference
xref: uniprot Q9VCJ8 17
xref: PTHR24260:SF115
organism: Drosophila melanogaster

SPZ s22 sa22 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SPZ
stoichiometric coefficient: 2.0

DISSOCIATION_LEFT__DL_dimer_phosphoCACT_UBI-RIGHT__DL-_CACT-_re38
type: ComplexAssembly
spontaneous: false
conversion direction: LEFT_TO_RIGHT
xref: DISSOCIATION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re38 151
participant stoichiometry: PARTICIPANT STOICHIOMETRY re38 153
participant stoichiometry: PARTICIPANT STOICHIOMETRY re38 152
left: DL dimer phosphoCACT UBI
interaction type: DISSOCIATION
right: DL
right: CACT

CACT s119 sa83 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: CACT
stoichiometric coefficient: 1.0

DIF
type: Protein

PARTICIPANT STOICHIOMETRY re17 168
type: Stoichiometry
physical entity: TL
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re17 169
type: Stoichiometry
physical entity: TL
stoichiometric coefficient: 2.0

TL
type: Protein
entity reference: TL
feature: ACTIVE 9

DIF
type: Protein

SPE s175 sa111 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SPE
stoichiometric coefficient: 1.0

eco ECO:0000316 15
type: UnificationXref
id: ECO:0000316
db: eco

HETERODIMER_ASSOCIATION_LEFT__SPZ-_TL-RIGHT__SPZ_dimer_TL-_re12
type: ComplexAssembly
spontaneous: true
conversion direction: LEFT_TO_RIGHT
xref: HETERODIMER ASSOCIATION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re12 184
participant stoichiometry: PARTICIPANT STOICHIOMETRY re12 186
participant stoichiometry: PARTICIPANT STOICHIOMETRY re12 185
left: TL
left: SPZ
interaction type: HETERODIMER_ASSOCIATION
right: SPZ dimer/TL

TUB s137 sa33 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: TUB
stoichiometric coefficient: 1.0

CACT
type: Protein
entity reference: CACT
feature: COMPLEX PARTICIPANT BINDING FEATURE s154 sa97 88
feature: CACT s154 sa97 rs1
feature: CACT s154 sa97 rs2
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

CATALYSIS
type: Catalysis
controlled: TRUNCATION_LEFT__SPZ_full_length-RIGHT__SPZ-_?-_re23
controller: SPE

SPZ s27 sa25 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SPZ
stoichiometric coefficient: 2.0

PTHR23199:SF4
type: RelationshipXref
id: PTHR23199:SF4
db: PANTHER Family
relationship type: By Homology

UNKNOWN_TRANSITION
type: InteractionVocabulary

PARTICIPANT STOICHIOMETRY re40 193
type: Stoichiometry
physical entity: DIF dimer phospho CACT
stoichiometric coefficient: 1.0

00696
type: UnificationXref
id: MOD:00696
db: mod

MyD88 s138 sa34 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: MyD88
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re40 192
type: Stoichiometry
physical entity: DIF dimer CACT
stoichiometric coefficient: 1.0

GO GO:0005829 3
type: UnificationXref
id: GO:0005829
db: GO

MyD88
type: Protein
entity reference: MyD88
feature: COMPLEX PARTICIPANT BINDING FEATURE s138 sa34 54
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

DL
type: Protein
entity reference: DL

MyD88/TUB
type: Complex
component stoichiometry: TUB s39 sa31 STOICHIOMETRY ID COMPLEX
component stoichiometry: MyD88 s40 sa32 STOICHIOMETRY ID COMPLEX
component: MyD88
component: TUB
feature: COMPLEX BINDING FEATURE s38 csa5 47
feature: COMPLEX BINDING FEATURE s38 csa5 49
cellular location: cytosol

DL
type: Protein
entity reference: DL

PLL s84 sa59 rs1
type: ModificationFeature
modification type: phosphorylated residue
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_NAME=
comment: MODIFICATION_RESIDUE_ANGLE=3.141592653589793

DIF s157 sa99 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DIF
stoichiometric coefficient: 2.0

HOMODIMER ID 11STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: TL
stoichiometric coefficient: 2.0

DISSOCIATION
type: RelationshipTypeVocabulary

PLL
type: Protein
entity reference: PLL
feature: ACTIVE 9
feature: PLL s84 sa59 rs1
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

BOOLEAN_LOGIC_GATE_OR
type: RelationshipTypeVocabulary

SPN27A
type: ProteinReference
xref: P06341
standard name: Serpin-27A
comment: ENTITY_REFERENCE_NOTES= serpin 27A Uniprot: Q9V3N1
comment: ENTITY_REFERENCE_PROTEIN_TYPE=GENERIC
comment: ENTITY_REFERENCE_ID_DESC=Toll_pathway_drosophila.PROTEIN.GENERIC.SPN27A
member entity reference: Serine protease inhibitor 27A

CACT
type: Protein
entity reference: CACT
feature: CACT s119 sa83 rs2
feature: COMPLEX PARTICIPANT BINDING FEATURE s119 sa83 94
feature: CACT s119 sa83 rs1
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

SPZ s74 sa50 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SPZ
stoichiometric coefficient: 2.0

DIF dimer phospho UBI CACT
type: Complex
component stoichiometry: CACT s167 sa106 STOICHIOMETRY ID COMPLEX
component stoichiometry: HOMODIMER ID 35STOICHIOMETRY ID COMPLEX
component: DIF
component: CACT
feature: COMPLEX BINDING FEATURE s166 csa23 71
feature: COMPLEX BINDING FEATURE s166 csa23 73
cellular location: cytosol

SPZ s139 sa58 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SPZ
stoichiometric coefficient: 2.0

PTHR11461:SF211
type: RelationshipXref
id: PTHR11461:SF211
db: PANTHER Family
relationship type: By Homology

STATE_TRANSITION_LEFT__SPZ_dimer__TL_dimer__MyD88_TUB__PLL-RIGHT__SPZ_dimer__TL_dimer__MyD88_TUB__PLL-_re21
type: BiochemicalReaction
conversion direction: LEFT_TO_RIGHT
xref: STATE TRANSITION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re21 210
participant stoichiometry: PARTICIPANT STOICHIOMETRY re21 209
left: SPZ dimer/ TL dimer/ MyD88/TUB/ PLL
interaction type: STATE_TRANSITION
right: SPZ dimer/ TL dimer/ MyD88/TUB/ PLL

CACT s158 sa100 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: CACT
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re28 197
type: Stoichiometry
physical entity: SLMB
stoichiometric coefficient: 1.0

SPZ full length
type: Protein
entity reference: SPZ full length

eco ECO:0000304 19
type: UnificationXref
id: ECO:0000304
db: eco

PARTICIPANT STOICHIOMETRY re28 198
type: Stoichiometry
physical entity: DL dimer phosphoCACT SLMB
stoichiometric coefficient: 1.0

SPZ
type: Protein
entity reference: SPZ

PARTICIPANT STOICHIOMETRY re28 196
type: Stoichiometry
physical entity: DL dimer phosphoCACT
stoichiometric coefficient: 1.0

SPZ
type: Protein
entity reference: SPZ

PARTICIPANT STOICHIOMETRY re27 199
type: Stoichiometry
physical entity: PLL
stoichiometric coefficient: 1.0

Embryonic polarity protein dorsal
type: ProteinReference
xref: PTHR24169:SF20
xref: uniprot P15330 21
organism: Drosophila melanogaster

PARTICIPANT STOICHIOMETRY re21 209
type: Stoichiometry
physical entity: SPZ dimer/ TL dimer/ MyD88/TUB/ PLL
stoichiometric coefficient: 1.0

STATE_TRANSITION_LEFT__TL-RIGHT__TL-_re17
type: BiochemicalReaction
conversion direction: LEFT_TO_RIGHT
xref: STATE TRANSITION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re17 168
participant stoichiometry: PARTICIPANT STOICHIOMETRY re17 169
left: TL
interaction type: STATE_TRANSITION
right: TL

STATE_TRANSITION
type: RelationshipTypeVocabulary

SLMB
type: Protein
entity reference: SLMB
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

SLMB
type: ProteinReference
xref: P06348
standard name: Slimb
comment: ENTITY_REFERENCE_NOTES= Slimb Uniprot: P44382
comment: ENTITY_REFERENCE_PROTEIN_TYPE=GENERIC
comment: ENTITY_REFERENCE_ID_DESC=Toll_pathway_drosophila.PROTEIN.GENERIC.SLMB
member entity reference: Supernumerary limbs, isoform B

PARTICIPANT STOICHIOMETRY re46 175
type: Stoichiometry
physical entity: SPN27A SPE
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re41 190
type: Stoichiometry
physical entity: SLMB
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re46 174
type: Stoichiometry
physical entity: SPN27A
stoichiometric coefficient: 1.0

TRUNCATION_LEFT__SPZ_full_length-RIGHT__SPZ-_?-_re23
type: BiochemicalReaction
conversion direction: LEFT_TO_RIGHT
xref: TRUNCATION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re23 204
participant stoichiometry: PARTICIPANT STOICHIOMETRY re23 203
participant stoichiometry: PARTICIPANT STOICHIOMETRY re23 205
left: SPZ full length
interaction type: TRUNCATION
right: SPZ
right: ?

PARTICIPANT STOICHIOMETRY re41 191
type: Stoichiometry
physical entity: DIF dimer/CACT SLMB
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re46 173
type: Stoichiometry
physical entity: SPE
stoichiometric coefficient: 1.0

SPN27A
type: Protein
entity reference: SPN27A
comment: SPECIES_TYPE=PROTEIN

DIF dimer CACT
type: Complex
component stoichiometry: CACT s151 sa96 STOICHIOMETRY ID COMPLEX
component stoichiometry: HOMODIMER ID 31STOICHIOMETRY ID COMPLEX
component: DIF
component: CACT
feature: COMPLEX BINDING FEATURE s152 csa19 67
feature: COMPLEX BINDING FEATURE s152 csa19 69
cellular location: cytosol

DL s94 sa64 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DL
stoichiometric coefficient: 2.0

PARTICIPANT STOICHIOMETRY re21 210
type: Stoichiometry
physical entity: SPZ dimer/ TL dimer/ MyD88/TUB/ PLL
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re41 189
type: Stoichiometry
physical entity: DIF dimer phospho CACT
stoichiometric coefficient: 1.0

DIF
type: Protein

DISSOCIATION_LEFT__DL_DIF_dimer_phosphoCACT_UBI_SLMB-RIGHT__DL_dimer_phosphoCACT_UBI-_SLMB-_re30
type: ComplexAssembly
spontaneous: false
conversion direction: LEFT_TO_RIGHT
xref: DISSOCIATION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re30 157
participant stoichiometry: PARTICIPANT STOICHIOMETRY re30 158
participant stoichiometry: PARTICIPANT STOICHIOMETRY re30 156
left: DL/DIF dimer phosphoCACT UBI SLMB
interaction type: DISSOCIATION
right: DL dimer phosphoCACT UBI
right: SLMB

PARTICIPANT STOICHIOMETRY re37 155
type: Stoichiometry
physical entity: sa94_degraded
stoichiometric coefficient: 1.0

uniprot P13582 16
type: UnificationXref
id: P13582
db: uniprot

PARTICIPANT STOICHIOMETRY re19 163
type: Stoichiometry
physical entity: PLL
stoichiometric coefficient: 1.0

DL dimer phosphoCACT SLMB
type: Complex
component stoichiometry: HOMODIMER ID 26STOICHIOMETRY ID COMPLEX
component stoichiometry: SLMB s109 sa74 STOICHIOMETRY ID COMPLEX
component stoichiometry: CACT s104 sa73 STOICHIOMETRY ID COMPLEX
component: DL
component: CACT
component: SLMB
feature: COMPLEX BINDING FEATURE s149 csa14 147
feature: COMPLEX BINDING FEATURE s149 csa14 149
feature: COMPLEX BINDING FEATURE s149 csa14 145
cellular location: cytosol

MyD88 s77 sa53 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: MyD88
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re19 162
type: Stoichiometry
physical entity: SPZ dimer/ TL dimer/ MyD88/TUB
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re37 154
type: Stoichiometry
physical entity: CACT
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re19 164
type: Stoichiometry
physical entity: SPZ dimer/ TL dimer/ MyD88/TUB/ PLL
stoichiometric coefficient: 1.0

CACT
type: ProteinReference
xref: P06342
standard name: cactus
comment: ENTITY_REFERENCE_PROTEIN_TYPE=GENERIC
comment: ENTITY_REFERENCE_ID_DESC=Toll_pathway_drosophila.PROTEIN.GENERIC.CACT
comment: ENTITY_REFERENCE_NOTES=
member entity reference: NF-kappa-B inhibitor cactus

DIF
type: Protein

CACT s167 sa106 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: CACT
stoichiometric coefficient: 1.0

IDA
type: EvidenceCodeVocabulary

PLL
type: Protein
entity reference: PLL
feature: PLL s53 sa39 rs1
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

TL s143 sa57 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: TL
stoichiometric coefficient: 2.0

MyD88
type: Protein
entity reference: MyD88
feature: COMPLEX PARTICIPANT BINDING FEATURE s138 sa56 62
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

HOMODIMER ID 34STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DIF
stoichiometric coefficient: 2.0

DL_dimer CACT
type: Complex
component stoichiometry: CACT s90 sa60 STOICHIOMETRY ID COMPLEX
component stoichiometry: HOMODIMER ID 24STOICHIOMETRY ID COMPLEX
component: DL
component: CACT
feature: COMPLEX BINDING FEATURE s147 csa12 77
feature: COMPLEX BINDING FEATURE s147 csa12 75
cellular location: cytosol

PARTICIPANT STOICHIOMETRY re25 202
type: Stoichiometry
physical entity: DL dimer phosphoCACT
stoichiometric coefficient: 1.0

SPE
type: Protein
entity reference: SPE
feature: COMPLEX PARTICIPANT BINDING FEATURE s175 sa111 108
comment: SPECIES_TYPE=PROTEIN

PARTICIPANT STOICHIOMETRY re25 201
type: Stoichiometry
physical entity: DL_dimer CACT
stoichiometric coefficient: 1.0

TL
type: Protein
entity reference: TL
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

SPZ
type: Protein
entity reference: SPZ

DL s128 sa89 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DL
stoichiometric coefficient: 2.0

HETERODIMER_ASSOCIATION_LEFT__MyD88-_TUB-RIGHT__MyD88_TUB-_re16
type: ComplexAssembly
spontaneous: true
conversion direction: LEFT_TO_RIGHT
xref: HETERODIMER ASSOCIATION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re16 172
participant stoichiometry: PARTICIPANT STOICHIOMETRY re16 170
participant stoichiometry: PARTICIPANT STOICHIOMETRY re16 171
left: MyD88
left: TUB
interaction type: HETERODIMER_ASSOCIATION
right: MyD88/TUB

EA
type: Protein
entity reference: EA
feature: COMPLEX PARTICIPANT BINDING FEATURE s20 sa19 86
comment: SPECIES_TYPE=PROTEIN

SPN27A
type: Protein
entity reference: SPN27A
feature: COMPLEX PARTICIPANT BINDING FEATURE s21 sa18 84
comment: SPECIES_TYPE=PROTEIN

DISSOCIATION_LEFT__SPZ_dimer__TL_dimer__MyD88_TUB__PLL-RIGHT__SPZ_dimer__TL_dimer__MyD88_TUB-_PLL-_re22
type: ComplexAssembly
spontaneous: false
conversion direction: LEFT_TO_RIGHT
xref: DISSOCIATION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re22 206
participant stoichiometry: PARTICIPANT STOICHIOMETRY re22 207
participant stoichiometry: PARTICIPANT STOICHIOMETRY re22 208
left: SPZ dimer/ TL dimer/ MyD88/TUB/ PLL
interaction type: DISSOCIATION
right: PLL
right: SPZ dimer/ TL dimer/ MyD88/TUB

UNKNOWN_TRANSITION
type: RelationshipTypeVocabulary

HOMODIMER ID 8STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: TL
stoichiometric coefficient: 2.0

CACT s163 sa102 rs1
type: ModificationFeature
modification type: phosphorylated residue
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_NAME=
comment: MODIFICATION_RESIDUE_ANGLE=3.141592653589793

PTHR24260:SF115
type: RelationshipXref
id: PTHR24260:SF115
db: PANTHER Family
relationship type: By Homology

HETERODIMER_ASSOCIATION_LEFT__EA-_SPN27A-RIGHT__EA_SPN27A-_re6
type: ComplexAssembly
spontaneous: true
conversion direction: LEFT_TO_RIGHT
xref: HETERODIMER ASSOCIATION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re6 159
participant stoichiometry: PARTICIPANT STOICHIOMETRY re6 161
participant stoichiometry: PARTICIPANT STOICHIOMETRY re6 160
left: EA
left: SPN27A
interaction type: HETERODIMER_ASSOCIATION
right: EA/SPN27A

HOMODIMER ID 27STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DL
stoichiometric coefficient: 2.0

TRANSPORT
type: RelationshipTypeVocabulary

PARTICIPANT STOICHIOMETRY re6 161
type: Stoichiometry
physical entity: EA/SPN27A
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re6 160
type: Stoichiometry
physical entity: SPN27A
stoichiometric coefficient: 1.0

TL
type: Complex
component stoichiometry: TL s143 sa35 STOICHIOMETRY ID COMPLEX
component: TL
feature: ACTIVE 9
feature: COMPLEX PARTICIPANT BINDING FEATURE s143 sa35 56
cellular location: cytosol

PARTICIPANT STOICHIOMETRY re27 200
type: Stoichiometry
physical entity: unknown protein
stoichiometric coefficient: 1.0

PLL
type: Protein
entity reference: PLL
feature: ACTIVE 9
feature: COMPLEX PARTICIPANT BINDING FEATURE s59 sa44 118
feature: PLL s59 sa44 rs1
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

HOMODIMER ID 28STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DL
stoichiometric coefficient: 2.0

DL s123 sa87 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DL
stoichiometric coefficient: 2.0

CACT s163 sa102 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: CACT
stoichiometric coefficient: 1.0

TUB
type: Protein
feature: ACTIVE 9
feature: COMPLEX PARTICIPANT BINDING FEATURE s76 sa52 124
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

DIF s161 sa103 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DIF
stoichiometric coefficient: 2.0

uniprot P15330 21
type: UnificationXref
id: P15330
db: uniprot

uniprot Q03017 20
type: UnificationXref
id: Q03017
db: uniprot

SPZ
type: ProteinReference
xref: P06349
standard name: Spatzle
comment: ENTITY_REFERENCE_NOTES= Protein spaetzle Uniprot: P48607
comment: ENTITY_REFERENCE_PROTEIN_TYPE=TRUNCATED
comment: ENTITY_REFERENCE_ID_DESC=Toll_pathway_drosophila.PROTEIN.TRUNCATED.SPZ
member entity reference: Protein spaetzle

CACT s114 sa78 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: CACT
stoichiometric coefficient: 1.0

CACT
type: Protein
entity reference: CACT
feature: CACT s134 sa94 rs1
feature: CACT s134 sa94 rs2
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

MODIFICATION FEATURE ubiquitinated
type: SequenceModificationVocabulary
comment: DATA_SOURCE=CellDesigner

SPN27A s21 sa18 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SPN27A
stoichiometric coefficient: 1.0

DL dimer phosphoCACT UBI
type: Complex
component stoichiometry: HOMODIMER ID 28STOICHIOMETRY ID COMPLEX
component stoichiometry: CACT s119 sa83 STOICHIOMETRY ID COMPLEX
component: DL
component: CACT
feature: COMPLEX BINDING FEATURE s150 csa16 91
feature: COMPLEX BINDING FEATURE s150 csa16 93
cellular location: cytosol

TUB s39 sa31 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: TUB
stoichiometric coefficient: 1.0

CACT s163 sa102 rs2
type: ModificationFeature
modification type: MODIFICATION FEATURE ubiquitinated
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_ANGLE=4.995363318667594
comment: MODIFICATION_RESIDUE_NAME=

HOMODIMER ID 44STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SPZ
stoichiometric coefficient: 2.0

TL s143 sa35 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: TL
stoichiometric coefficient: 2.0

phosphorylated residue
type: SequenceModificationVocabulary
xref: 00696
comment: DATA_SOURCE=CellDesigner

SPZ s139 sa36 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SPZ
stoichiometric coefficient: 2.0

TL
type: Protein
entity reference: TL
feature: ACTIVE 9

PARTICIPANT STOICHIOMETRY re6 159
type: Stoichiometry
physical entity: EA
stoichiometric coefficient: 1.0

uniprot Q9V3N1 18
type: UnificationXref
id: Q9V3N1
db: uniprot

CACT
type: Protein
entity reference: CACT
feature: CACT s151 sa96 rs1
feature: COMPLEX PARTICIPANT BINDING FEATURE s151 sa96 70
feature: CACT s151 sa96 rs2
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

Serine protease easter
type: ProteinReference
xref: PTHR24256:SF449
xref: uniprot P13582 16
organism: Drosophila melanogaster

PLL s78 sa54 rs1
type: ModificationFeature
modification type: phosphorylated residue
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_NAME=
comment: MODIFICATION_RESIDUE_ANGLE=3.141592653589793

target genes
type: DnaRegion
cellular location: nucleus
comment: SPECIES_TYPE=GENE

TUB s76 sa52 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: TUB
stoichiometric coefficient: 1.0

MyD88 s57 sa43 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: MyD88
stoichiometric coefficient: 1.0

DL
type: Protein
entity reference: DL

SPZ dimer/ TL dimer/ MyD88/TUB/ PLL
type: Complex
component stoichiometry: TUB s76 sa52 STOICHIOMETRY ID COMPLEX
component stoichiometry: MyD88 s77 sa53 STOICHIOMETRY ID COMPLEX
component stoichiometry: PLL s78 sa54 STOICHIOMETRY ID COMPLEX
component stoichiometry: HOMODIMER ID 44STOICHIOMETRY ID COMPLEX
component stoichiometry: HOMODIMER ID 13STOICHIOMETRY ID COMPLEX
component: PLL
component: TUB
component: MyD88
component: TL
component: SPZ
feature: COMPLEX BINDING FEATURE s73 csa10 121
feature: COMPLEX BINDING FEATURE s73 csa10 125
feature: COMPLEX BINDING FEATURE s73 csa10 123
feature: COMPLEX BINDING FEATURE s73 csa10 127
feature: COMPLEX BINDING FEATURE s73 csa10 119
cellular location: cytosol

HETERODIMER_ASSOCIATION_LEFT__SPZ_dimer__TL_dimer__MyD88_TUB-_PLL-RIGHT__SPZ_dimer__TL_dimer__MyD88_TUB__PLL-_re19
type: ComplexAssembly
spontaneous: true
conversion direction: LEFT_TO_RIGHT
xref: HETERODIMER ASSOCIATION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re19 162
participant stoichiometry: PARTICIPANT STOICHIOMETRY re19 164
participant stoichiometry: PARTICIPANT STOICHIOMETRY re19 163
left: SPZ dimer/ TL dimer/ MyD88/TUB
left: PLL
interaction type: HETERODIMER_ASSOCIATION
right: SPZ dimer/ TL dimer/ MyD88/TUB/ PLL

DISSOCIATION
type: InteractionVocabulary

Serine protease inhibitor 27A
type: ProteinReference
xref: PTHR11461:SF211
xref: uniprot Q9V3N1 18
organism: Drosophila melanogaster

TL
type: Protein
entity reference: TL
feature: ACTIVE 9

TL s145 sa41 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: TL
stoichiometric coefficient: 2.0

DL/DIF dimer phosphoCACT UBI SLMB
type: Complex
component stoichiometry: SLMB s113 sa77 STOICHIOMETRY ID COMPLEX
component stoichiometry: CACT s114 sa78 STOICHIOMETRY ID COMPLEX
component stoichiometry: HOMODIMER ID 27STOICHIOMETRY ID COMPLEX
component: DL
component: CACT
component: SLMB
feature: COMPLEX BINDING FEATURE s115 csa15 135
feature: COMPLEX BINDING FEATURE s115 csa15 137
feature: COMPLEX BINDING FEATURE s115 csa15 133
cellular location: cytosol

CACT s154 sa97 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: CACT
stoichiometric coefficient: 1.0

CATALYSIS
type: Catalysis
controlled: STATE_TRANSITION_LEFT__DL_dimer_CACT-RIGHT__DL_dimer_phosphoCACT-_re25
controller: unknown protein

SPN27A SPE
type: Complex
component stoichiometry: SPN27A s174 sa110 STOICHIOMETRY ID COMPLEX
component stoichiometry: SPE s175 sa111 STOICHIOMETRY ID COMPLEX
component: SPE
component: SPN27A
feature: COMPLEX BINDING FEATURE s173 csa24 105
feature: COMPLEX BINDING FEATURE s173 csa24 107

DL s118 sa81 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DL
stoichiometric coefficient: 2.0

DIF dimer phosphoCACT UBI SLMB
type: Complex
component stoichiometry: CACT s163 sa102 STOICHIOMETRY ID COMPLEX
component stoichiometry: HOMODIMER ID 34STOICHIOMETRY ID COMPLEX
component stoichiometry: SLMB s164 sa104 STOICHIOMETRY ID COMPLEX
component: DIF
component: CACT
component: SLMB
feature: COMPLEX BINDING FEATURE s162 csa22 103
feature: COMPLEX BINDING FEATURE s162 csa22 99
feature: COMPLEX BINDING FEATURE s162 csa22 101
cellular location: cytosol

PTHR24169:SF20
type: RelationshipXref
id: PTHR24169:SF20
db: PANTHER Family
relationship type: By Homology

SPE
type: ProteinReference
xref: P06340
standard name: Spatzle-processing enzyme
comment: ENTITY_REFERENCE_NOTES= Uniprot: Q9VCJ8 Flybase: CG16705
comment: ENTITY_REFERENCE_PROTEIN_TYPE=GENERIC
comment: ENTITY_REFERENCE_ID_DESC=Toll_pathway_drosophila.PROTEIN.GENERIC.SPE
member entity reference: Spaetzle-processing enzyme

PARTICIPANT STOICHIOMETRY re39 212
type: Stoichiometry
physical entity: DL
stoichiometric coefficient: 2.0

GO GO:0005634 2
type: UnificationXref
id: GO:0005634
db: GO

PARTICIPANT STOICHIOMETRY re39 211
type: Stoichiometry
physical entity: DL
stoichiometric coefficient: 2.0

CACT s134 sa94 rs2
type: ModificationFeature
modification type: MODIFICATION FEATURE ubiquitinated
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_ANGLE=4.995363318667594
comment: MODIFICATION_RESIDUE_NAME=

CACT s158 sa100 rs2
type: ModificationFeature
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_ANGLE=4.995363318667594
comment: MODIFICATION_RESIDUE_NAME=

PARTICIPANT STOICHIOMETRY re30 157
type: Stoichiometry
physical entity: DL dimer phosphoCACT UBI
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re30 156
type: Stoichiometry
physical entity: DL/DIF dimer phosphoCACT UBI SLMB
stoichiometric coefficient: 1.0

CACT
type: Protein
entity reference: CACT
feature: CACT s114 sa78 rs2
feature: CACT s114 sa78 rs1
feature: COMPLEX PARTICIPANT BINDING FEATURE s114 sa78 138
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

PLL s59 sa44 rs1
type: ModificationFeature
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_NAME=
comment: MODIFICATION_RESIDUE_ANGLE=3.141592653589793

DISSOCIATION_LEFT__DIF_dimer_phospho_UBI_CACT-RIGHT__DIF-_CACT-_re44
type: ComplexAssembly
spontaneous: false
conversion direction: LEFT_TO_RIGHT
xref: DISSOCIATION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re44 178
participant stoichiometry: PARTICIPANT STOICHIOMETRY re44 179
participant stoichiometry: PARTICIPANT STOICHIOMETRY re44 180
left: DIF dimer phospho UBI CACT
interaction type: DISSOCIATION
right: DIF
right: CACT

Supernumerary limbs, isoform B
type: ProteinReference
xref: PTHR44156:SF6
xref: uniprot A0A0B4KHK1 39
organism: Drosophila melanogaster

PARTICIPANT STOICHIOMETRY re30 158
type: Stoichiometry
physical entity: SLMB
stoichiometric coefficient: 1.0

DL
type: Protein
entity reference: DL

CACT
type: Protein
entity reference: CACT
feature: CACT s90 sa60 rs2
feature: CACT s90 sa60 rs1
feature: COMPLEX PARTICIPANT BINDING FEATURE s90 sa60 76
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

TUB s137 sa55 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: TUB
stoichiometric coefficient: 1.0

STATE_TRANSITION_LEFT__DIF_dimer_CACT-RIGHT__DIF_dimer_phospho_CACT-_re40
type: BiochemicalReaction
conversion direction: LEFT_TO_RIGHT
xref: STATE TRANSITION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re40 193
participant stoichiometry: PARTICIPANT STOICHIOMETRY re40 192
left: DIF dimer CACT
interaction type: STATE_TRANSITION
right: DIF dimer phospho CACT

CACT s158 sa100 rs1
type: ModificationFeature
modification type: phosphorylated residue
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_NAME=
comment: MODIFICATION_RESIDUE_ANGLE=3.141592653589793

DL
type: Protein
entity reference: DL

CACT s134 sa94 rs1
type: ModificationFeature
modification type: phosphorylated residue
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_NAME=
comment: MODIFICATION_RESIDUE_ANGLE=3.141592653589793

DIF s169 sa108 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DIF
stoichiometric coefficient: 2.0

SPE
type: Protein
entity reference: SPE
feature: ACTIVE 9
comment: SPECIES_TYPE=PROTEIN

CATALYSIS
type: Catalysis
controlled: TRUNCATION_LEFT__SPZ_full_length-RIGHT__SPZ-_?-_re23
controller: EA

Protein spaetzle
type: ProteinReference
xref: uniprot P48607 37
xref: PTHR23199:SF4
organism: Drosophila melanogaster

PLL s78 sa54 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: PLL
stoichiometric coefficient: 1.0

SLMB
type: Protein
entity reference: SLMB
feature: COMPLEX PARTICIPANT BINDING FEATURE s164 sa104 104
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

sa94_degraded
type: PhysicalEntity
cellular location: cytosol
comment: SPECIES_TYPE=DEGRADED

DIF dimer/CACT SLMB
type: Complex
component stoichiometry: HOMODIMER ID 33STOICHIOMETRY ID COMPLEX
component stoichiometry: SLMB s160 sa101 STOICHIOMETRY ID COMPLEX
component stoichiometry: CACT s158 sa100 STOICHIOMETRY ID COMPLEX
component: CACT
component: SLMB
component: DIF
feature: COMPLEX BINDING FEATURE s159 csa21 143
feature: COMPLEX BINDING FEATURE s159 csa21 141
feature: COMPLEX BINDING FEATURE s159 csa21 139
cellular location: cytosol

uniprot A0A0B4KHK1 39
type: UnificationXref
id: A0A0B4KHK1
db: uniprot

SPZ
type: Complex
component stoichiometry: SPZ s58 sa40 STOICHIOMETRY ID COMPLEX
component: SPZ
feature: COMPLEX PARTICIPANT BINDING FEATURE s58 sa40 110
cellular location: cytosol

SPZ
type: Complex
component stoichiometry: SPZ s51 sa38 STOICHIOMETRY ID COMPLEX
component: SPZ
feature: COMPLEX PARTICIPANT BINDING FEATURE s51 sa38 82
cellular location: cytosol

SPZ
type: Complex
component stoichiometry: SPZ s27 sa25 STOICHIOMETRY ID COMPLEX
component: SPZ
feature: COMPLEX PARTICIPANT BINDING FEATURE s27 sa25 98
cellular location: cytosol

SPZ
type: Complex
component stoichiometry: SPZ s22 sa22 STOICHIOMETRY ID COMPLEX
component: SPZ

SPZ full length
type: ProteinReference
xref: P06347
standard name: Spatzle
comment: ENTITY_REFERENCE_PROTEIN_TYPE=GENERIC
comment: ENTITY_REFERENCE_NOTES= Protein spaetzle Uniprot: P48607
comment: ENTITY_REFERENCE_ID_DESC=Toll_pathway_drosophila.PROTEIN.GENERIC.SPZ_br_full length
member entity reference: Protein spaetzle

PARTICIPANT STOICHIOMETRY re45 177
type: Stoichiometry
physical entity: DIF
stoichiometric coefficient: 2.0

PARTICIPANT STOICHIOMETRY re45 176
type: Stoichiometry
physical entity: DIF
stoichiometric coefficient: 2.0

PTHR46680:SF1
type: RelationshipXref
id: PTHR46680:SF1
db: PANTHER Family
relationship type: By Homology

MyD88
type: ProteinReference
xref: P06346
comment: ENTITY_REFERENCE_PROTEIN_TYPE=GENERIC
comment: ENTITY_REFERENCE_NOTES= MyD88 addaptor protein Uniprot: Q7K105
comment: ENTITY_REFERENCE_ID_DESC=Toll_pathway_drosophila.PROTEIN.GENERIC.MyD88
member entity reference: LD20892p

DIF
type: Complex
component stoichiometry: DIF s165 sa105 STOICHIOMETRY ID COMPLEX
component: DIF
feature: COMPLEX PARTICIPANT BINDING FEATURE s165 sa105 72
cellular location: cytosol

DIF
type: Complex
component stoichiometry: DIF s157 sa99 STOICHIOMETRY ID COMPLEX
component: DIF
feature: COMPLEX PARTICIPANT BINDING FEATURE s157 sa99 140
cellular location: cytosol

DIF
type: Complex
component stoichiometry: DIF s161 sa103 STOICHIOMETRY ID COMPLEX
component: DIF
feature: COMPLEX PARTICIPANT BINDING FEATURE s161 sa103 102
cellular location: cytosol

HOMODIMER ID 45STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SPZ
stoichiometric coefficient: 2.0

SLMB s160 sa101 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SLMB
stoichiometric coefficient: 1.0

SPZ full length
type: Complex
component stoichiometry: SPZ full length s23 sa23 STOICHIOMETRY ID COMPLEX
component: SPZ full length

CACT s96 sa63 rs1
type: ModificationFeature
modification type: phosphorylated residue
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_NAME=
comment: MODIFICATION_RESIDUE_ANGLE=3.141592653589793

CACT s119 sa83 rs2
type: ModificationFeature
modification type: MODIFICATION FEATURE ubiquitinated
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_ANGLE=4.995363318667594
comment: MODIFICATION_RESIDUE_NAME=

HOMODIMER ID 13STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: TL
stoichiometric coefficient: 2.0

STATE_TRANSITION_LEFT__CACT-RIGHT__sa94_degraded-_re37
type: Degradation
conversion direction: LEFT_TO_RIGHT
xref: STATE TRANSITION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re37 155
participant stoichiometry: PARTICIPANT STOICHIOMETRY re37 154
left: CACT
interaction type: STATE_TRANSITION
right: sa94_degraded

HETERODIMER_ASSOCIATION
type: InteractionVocabulary

HOMODIMER ID 26STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DL
stoichiometric coefficient: 2.0

CACT
type: Protein
entity reference: CACT
feature: CACT s96 sa63 rs1
feature: CACT s96 sa63 rs2
feature: COMPLEX PARTICIPANT BINDING FEATURE s96 sa63 130
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

SPZ
type: Protein
entity reference: SPZ

TL s144 sa37 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: TL
stoichiometric coefficient: 2.0

SPZ dimer/TL
type: Complex
component stoichiometry: HOMODIMER ID 41STOICHIOMETRY ID COMPLEX
component stoichiometry: TL s142 sa24 STOICHIOMETRY ID COMPLEX
component: SPZ
component: TL
feature: COMPLEX BINDING FEATURE s26 csa2 97
feature: COMPLEX BINDING FEATURE s26 csa2 95
cellular location: cytosol

SPZ
type: Complex
component stoichiometry: SPZ s74 sa50 STOICHIOMETRY ID COMPLEX
component: SPZ
feature: COMPLEX PARTICIPANT BINDING FEATURE s74 sa50 120
cellular location: cytosol

SPZ
type: Complex
component stoichiometry: SPZ s139 sa36 STOICHIOMETRY ID COMPLEX
component: SPZ
feature: COMPLEX PARTICIPANT BINDING FEATURE s139 sa36 58
cellular location: cytosol

SPZ
type: Complex
component stoichiometry: SPZ s139 sa58 STOICHIOMETRY ID COMPLEX
component: SPZ
feature: COMPLEX PARTICIPANT BINDING FEATURE s139 sa58 66
cellular location: cytosol

TAS
type: EvidenceCodeVocabulary
xref: eco ECO:0000304 19

SPZ dimer/ TL dimer/ MyD88/TUB/ PLL
type: Complex
component stoichiometry: HOMODIMER ID 43STOICHIOMETRY ID COMPLEX
component stoichiometry: TUB s56 sa42 STOICHIOMETRY ID COMPLEX
component stoichiometry: MyD88 s57 sa43 STOICHIOMETRY ID COMPLEX
component stoichiometry: HOMODIMER ID 12STOICHIOMETRY ID COMPLEX
component stoichiometry: PLL s59 sa44 STOICHIOMETRY ID COMPLEX
component: PLL
component: SPZ
component: TUB
component: TL
component: MyD88
feature: COMPLEX BINDING FEATURE s55 csa8 111
feature: COMPLEX BINDING FEATURE s55 csa8 109
feature: COMPLEX BINDING FEATURE s55 csa8 115
feature: COMPLEX BINDING FEATURE s55 csa8 113
feature: COMPLEX BINDING FEATURE s55 csa8 117
cellular location: cytosol

CACT s119 sa83 rs1
type: ModificationFeature
modification type: phosphorylated residue
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_NAME=
comment: MODIFICATION_RESIDUE_ANGLE=3.141592653589793

CACT s96 sa63 rs2
type: ModificationFeature
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_ANGLE=4.995363318667594
comment: MODIFICATION_RESIDUE_NAME=

CATALYSIS
type: Catalysis
controlled: STATE_TRANSITION_LEFT__DL_dimer_phosphoCACT_SLMB-RIGHT__DL_DIF_dimer_phosphoCACT_UBI_SLMB-_re29
controller: SLMB

CACT s104 sa73 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: CACT
stoichiometric coefficient: 1.0

MyD88
type: Protein
entity reference: MyD88
feature: COMPLEX PARTICIPANT BINDING FEATURE s57 sa43 116
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

HETERODIMER_ASSOCIATION_LEFT__DL_dimer_phosphoCACT-_SLMB-RIGHT__DL_dimer_phosphoCACT_SLMB-_re28
type: ComplexAssembly
spontaneous: true
conversion direction: LEFT_TO_RIGHT
xref: HETERODIMER ASSOCIATION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re28 196
participant stoichiometry: PARTICIPANT STOICHIOMETRY re28 197
participant stoichiometry: PARTICIPANT STOICHIOMETRY re28 198
left: SLMB
left: DL dimer phosphoCACT
interaction type: HETERODIMER_ASSOCIATION
right: DL dimer phosphoCACT SLMB

DISSOCIATION_LEFT__DIF_dimer_phosphoCACT_UBI_SLMB-RIGHT__DIF_dimer_phospho_UBI_CACT-_SLMB-_re43
type: ComplexAssembly
spontaneous: false
conversion direction: LEFT_TO_RIGHT
xref: DISSOCIATION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re43 183
participant stoichiometry: PARTICIPANT STOICHIOMETRY re43 182
participant stoichiometry: PARTICIPANT STOICHIOMETRY re43 181
left: DIF dimer phosphoCACT UBI SLMB
interaction type: DISSOCIATION
right: DIF dimer phospho UBI CACT
right: SLMB

PTHR24365:SF537
type: RelationshipXref
id: PTHR24365:SF537
db: PANTHER Family
relationship type: By Homology

SPZ dimer/ TL dimer/ MyD88/TUB
type: Complex
component stoichiometry: HOMODIMER ID 46STOICHIOMETRY ID COMPLEX
component stoichiometry: MyD88 s138 sa56 STOICHIOMETRY ID COMPLEX
component stoichiometry: HOMODIMER ID 10STOICHIOMETRY ID COMPLEX
component stoichiometry: TUB s137 sa55 STOICHIOMETRY ID COMPLEX
component: TUB
component: TL
component: MyD88
component: SPZ
feature: COMPLEX BINDING FEATURE s136 csa11 65
feature: COMPLEX BINDING FEATURE s136 csa11 63
feature: COMPLEX BINDING FEATURE s136 csa11 61
feature: COMPLEX BINDING FEATURE s136 csa11 59
cellular location: cytosol

MyD88
type: Protein
entity reference: MyD88
feature: COMPLEX PARTICIPANT BINDING FEATURE s40 sa32 50
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

HETERODIMER_ASSOCIATION_LEFT__MyD88_TUB-_SPZ_dimer__TL_dimer-RIGHT__SPZ_dimer__TL_dimer__MyD88_TUB-_re18
type: ComplexAssembly
spontaneous: true
conversion direction: LEFT_TO_RIGHT
xref: HETERODIMER ASSOCIATION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re18 165
participant stoichiometry: PARTICIPANT STOICHIOMETRY re18 166
participant stoichiometry: PARTICIPANT STOICHIOMETRY re18 167
left: SPZ dimer/ TL dimer
left: MyD88/TUB
interaction type: HETERODIMER_ASSOCIATION
right: SPZ dimer/ TL dimer/ MyD88/TUB

PTHR24419:SF33
type: RelationshipXref
id: PTHR24419:SF33
db: PANTHER Family
relationship type: By Homology

BOOLEAN_LOGIC_GATE_OR
type: Control
xref: BOOLEAN LOGIC GATE OR
controlled: TRUNCATION_LEFT__SPZ_full_length-RIGHT__SPZ-_?-_re23

Serine/threonine-protein kinase pelle
type: ProteinReference
xref: uniprot Q05652 14
xref: PTHR24419:SF33
organism: Drosophila melanogaster

PARTICIPANT STOICHIOMETRY re12 186
type: Stoichiometry
physical entity: SPZ dimer/TL
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re12 185
type: Stoichiometry
physical entity: TL
stoichiometric coefficient: 1.0

PARTICIPANT STOICHIOMETRY re12 184
type: Stoichiometry
physical entity: SPZ
stoichiometric coefficient: 2.0

CACT
type: Protein
entity reference: CACT
feature: CACT s104 sa73 rs2
feature: COMPLEX PARTICIPANT BINDING FEATURE s104 sa73 148
feature: CACT s104 sa73 rs1
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

CACT s151 sa96 rs1
type: ModificationFeature
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_NAME=
comment: MODIFICATION_RESIDUE_ANGLE=3.141592653589793

CACT s151 sa96 rs2
type: ModificationFeature
comment: MODIFICATION_RESIDUE_SIDE=none
comment: MODIFICATION_RESIDUE_TYPE=residue
comment: MODIFICATION_RESIDUE_ANGLE=4.995363318667594
comment: MODIFICATION_RESIDUE_NAME=

SLMB
type: Protein
entity reference: SLMB
feature: COMPLEX PARTICIPANT BINDING FEATURE s113 sa77 136
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

SLMB s164 sa104 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SLMB
stoichiometric coefficient: 1.0

SPZ full length s23 sa23 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SPZ full length
stoichiometric coefficient: 2.0

TUB
type: Protein
feature: COMPLEX PARTICIPANT BINDING FEATURE s137 sa33 52
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

MyD88
type: Protein
entity reference: MyD88
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

HOMODIMER ID 24STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DL
stoichiometric coefficient: 2.0

HOMODIMER ID 33STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DIF
stoichiometric coefficient: 2.0

Toll_pathway_drosophila
type: Pathway
pathway component: UNKNOWN_TRANSITION_LEFT__PLL-RIGHT__unknown_protein-_re27
pathway component: HETERODIMER_ASSOCIATION_LEFT__SPZ-_TL-RIGHT__SPZ_dimer_TL-_re12
pathway component: STATE_TRANSITION_LEFT__DL_dimer_phosphoCACT_SLMB-RIGHT__DL_DIF_dimer_phosphoCACT_UBI_SLMB-_re29
pathway component: HETERODIMER_ASSOCIATION_LEFT__SPE-_SPN27A-RIGHT__SPN27A_SPE-_re46
pathway component: DISSOCIATION_LEFT__DIF_dimer_phosphoCACT_UBI_SLMB-RIGHT__DIF_dimer_phospho_UBI_CACT-_SLMB-_re43
pathway component: HETERODIMER_ASSOCIATION_LEFT__DIF_dimer_phospho_CACT-_SLMB-RIGHT__DIF_dimer_CACT_SLMB-_re41
pathway component: STATE_TRANSITION_LEFT__DIF_dimer_CACT_SLMB-RIGHT__DIF_dimer_phosphoCACT_UBI_SLMB-_re42
pathway component: HETERODIMER_ASSOCIATION_LEFT__SPZ_dimer__TL_dimer__MyD88_TUB-_PLL-RIGHT__SPZ_dimer__TL_dimer__MyD88_TUB__PLL-_re19
pathway component: HETERODIMER_ASSOCIATION_LEFT__MyD88-_TUB-RIGHT__MyD88_TUB-_re16
pathway component: DISSOCIATION_LEFT__DL_dimer_phosphoCACT_UBI-RIGHT__DL-_CACT-_re38
pathway component: TRUNCATION_LEFT__SPZ_full_length-RIGHT__SPZ-_?-_re23
pathway component: STATE_TRANSITION_LEFT__DIF_dimer_CACT-RIGHT__DIF_dimer_phospho_CACT-_re40
pathway component: TRANSPORT_LEFT__DL-RIGHT__DL-_re39
pathway component: STATE_TRANSITION_LEFT__SPZ_dimer__TL_dimer__MyD88_TUB__PLL-RIGHT__SPZ_dimer__TL_dimer__MyD88_TUB__PLL-_re21
pathway component: DISSOCIATION_LEFT__SPZ_dimer__TL_dimer__MyD88_TUB__PLL-RIGHT__SPZ_dimer__TL_dimer__MyD88_TUB-_PLL-_re22
pathway component: TRANSPORT_LEFT__DIF-RIGHT__DIF-_re45
pathway component: STATE_TRANSITION_LEFT__CACT-RIGHT__sa94_degraded-_re37
pathway component: DISSOCIATION_LEFT__DL_DIF_dimer_phosphoCACT_UBI_SLMB-RIGHT__DL_dimer_phosphoCACT_UBI-_SLMB-_re30
pathway component: HETERODIMER_ASSOCIATION_LEFT__EA-_SPN27A-RIGHT__EA_SPN27A-_re6
pathway component: HETERODIMER_ASSOCIATION_LEFT__DL_dimer_phosphoCACT-_SLMB-RIGHT__DL_dimer_phosphoCACT_SLMB-_re28
pathway component: STATE_TRANSITION_LEFT__DL_dimer_CACT-RIGHT__DL_dimer_phosphoCACT-_re25
pathway component: STATE_TRANSITION_LEFT__TL-RIGHT__TL-_re17
pathway component: HETERODIMER_ASSOCIATION_LEFT__MyD88_TUB-_SPZ_dimer__TL_dimer-RIGHT__SPZ_dimer__TL_dimer__MyD88_TUB-_re18
pathway component: DISSOCIATION_LEFT__DIF_dimer_phospho_UBI_CACT-RIGHT__DIF-_CACT-_re44
xref: PANTHER Pathway P06217 1
comment: MODEL_NOTES= In Drosophila, the Toll pathway establishes the embryonic dorsoventral axis and triggers innate immune responses to infection, along with the Imd pathway. In response to Gram-positive bacterial and fungal pathogens, the Toll pathway is activated. Lysine-type peptidoglycans, present in many Gram-positive bacteria, interact with soluble receptors PGRP-SA and PGRP-SD and trigger a serine protease cascade which results in the enzymatic processing of the full-length Spatzle (SPZ) dimer ligand. The final serine protease in the cascade is believed to be Easter (EA) in dorsoventral formation and Spatzle-processing enzyme aka SPE (CG16705) in an innate immunity response. The processed SPZ dimer binds to the extracellular part of the Toll (TL) receptor at the plasma membrane. This causes TL to activate and dimerise through its cytoplasmic domains. In response to this activity, the adaptor proteins MYD88, Tube (TUB), and the Ser/Thr kinase Pelle (PLL) are recruited to the TL receptor cytoplasmic region to form the ‘signalling complex’. PLL is activated, autophosphorylates and then dissociates from the ‘signalling complex’. The next stage is unclear but it is believed that PLL and an unknown kinase tentatively labelled the ‘Cactus kinase’ phosphorylate NF-kappaB inhibitor orthologue, Cactus (CACT). CACT is complexed to NF-kappaB orthologue dimers of either Dorsal (DL) in dorsoventral polarity or Dorsal-related immunity factor, Dif (DIF) in the innate immunity response. The phosphorylated CACT is ubiquitinated and degraded by the 26S proteasome, releasing the free DL/DIF dimer. This translocates to the nucleus where it activates transcription of genes encoding a battery of antimicrobial peptides in the immune response or genes that organise dorsoventral patterning.

PARTICIPANT STOICHIOMETRY re22 206
type: Stoichiometry
physical entity: SPZ dimer/ TL dimer/ MyD88/TUB/ PLL
stoichiometric coefficient: 1.0

STATE_TRANSITION
type: InteractionVocabulary

PARTICIPANT STOICHIOMETRY re22 208
type: Stoichiometry
physical entity: PLL
stoichiometric coefficient: 1.0

HOMODIMER ID 25STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DL
stoichiometric coefficient: 2.0

PARTICIPANT STOICHIOMETRY re22 207
type: Stoichiometry
physical entity: SPZ dimer/ TL dimer/ MyD88/TUB
stoichiometric coefficient: 1.0

MyD88 s40 sa32 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: MyD88
stoichiometric coefficient: 1.0

TRANSPORT
type: InteractionVocabulary

HETERODIMER_ASSOCIATION_LEFT__SPE-_SPN27A-RIGHT__SPN27A_SPE-_re46
type: ComplexAssembly
spontaneous: true
conversion direction: LEFT_TO_RIGHT
xref: HETERODIMER ASSOCIATION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re46 173
participant stoichiometry: PARTICIPANT STOICHIOMETRY re46 174
participant stoichiometry: PARTICIPANT STOICHIOMETRY re46 175
left: SPE
left: SPN27A
interaction type: HETERODIMER_ASSOCIATION
right: SPN27A SPE

DIF s153 sa62 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DIF
stoichiometric coefficient: 2.0

TRUNCATION
type: InteractionVocabulary

SPN27A s174 sa110 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SPN27A
stoichiometric coefficient: 1.0

TUB s56 sa42 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: TUB
stoichiometric coefficient: 1.0

DL s103 sa72 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DL
stoichiometric coefficient: 2.0

HOMODIMER ID 42STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: SPZ
stoichiometric coefficient: 2.0

EA/SPN27A
type: Complex
component stoichiometry: SPN27A s21 sa18 STOICHIOMETRY ID COMPLEX
component stoichiometry: EA s20 sa19 STOICHIOMETRY ID COMPLEX
component: SPN27A
component: EA
feature: COMPLEX BINDING FEATURE s19 csa1 85
feature: COMPLEX BINDING FEATURE s19 csa1 83

TL
type: Complex
component stoichiometry: TL s143 sa57 STOICHIOMETRY ID COMPLEX
component: TL
feature: ACTIVE 9
feature: COMPLEX PARTICIPANT BINDING FEATURE s143 sa57 64
cellular location: cytosol

CACT s90 sa60 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: CACT
stoichiometric coefficient: 1.0

SPZ
type: Protein
entity reference: SPZ

DIF s165 sa105 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DIF
stoichiometric coefficient: 2.0

unknown protein
type: Protein
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

DIF s155 sa98 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DIF
stoichiometric coefficient: 2.0

EA
type: ProteinReference
xref: P06339
standard name: Serine protease easter
comment: ENTITY_REFERENCE_PROTEIN_TYPE=GENERIC
comment: ENTITY_REFERENCE_NOTES= serine protease easter Uniprot: P13582 Uniprot: Q9VCJ8? (CG16705, SPE)
comment: ENTITY_REFERENCE_ID_DESC=Toll_pathway_drosophila.PROTEIN.GENERIC.EA
member entity reference: Serine protease easter

STATE_TRANSITION_LEFT__DL_dimer_phosphoCACT_SLMB-RIGHT__DL_DIF_dimer_phosphoCACT_UBI_SLMB-_re29
type: BiochemicalReaction
conversion direction: LEFT_TO_RIGHT
xref: STATE TRANSITION
participant stoichiometry: PARTICIPANT STOICHIOMETRY re29 195
participant stoichiometry: PARTICIPANT STOICHIOMETRY re29 194
left: DL dimer phosphoCACT SLMB
interaction type: STATE_TRANSITION
right: DL/DIF dimer phosphoCACT UBI SLMB

cytosol
type: CellularLocationVocabulary
xref: GO GO:0005829 3

IGI
type: EvidenceCodeVocabulary
xref: eco ECO:0000316 15

TL
type: Complex
component stoichiometry: TL s146 sa51 STOICHIOMETRY ID COMPLEX
component: TL
feature: COMPLEX PARTICIPANT BINDING FEATURE s146 sa51 122
feature: ACTIVE 9
cellular location: cytosol

TL
type: Complex
component stoichiometry: TL s145 sa41 STOICHIOMETRY ID COMPLEX
component: TL
feature: COMPLEX PARTICIPANT BINDING FEATURE s145 sa41 112
feature: ACTIVE 9
cellular location: cytosol

TL
type: Complex
component stoichiometry: TL s144 sa37 STOICHIOMETRY ID COMPLEX
component: TL
feature: ACTIVE 9
feature: COMPLEX PARTICIPANT BINDING FEATURE s144 sa37 80
cellular location: cytosol

CACT
type: Protein
entity reference: CACT
feature: COMPLEX PARTICIPANT BINDING FEATURE s163 sa102 100
feature: CACT s163 sa102 rs2
feature: CACT s163 sa102 rs1
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

TUB
type: Protein
feature: ACTIVE 9
feature: COMPLEX PARTICIPANT BINDING FEATURE s56 sa42 114
cellular location: cytosol
comment: SPECIES_TYPE=PROTEIN

HOMODIMER ID 31STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: DIF
stoichiometric coefficient: 2.0

HETERODIMER_ASSOCIATION
type: RelationshipTypeVocabulary

DIF
type: Complex
component stoichiometry: DIF s169 sa108 STOICHIOMETRY ID COMPLEX
component: DIF
cellular location: nucleus

PARTICIPANT STOICHIOMETRY re29 195
type: Stoichiometry
physical entity: DL/DIF dimer phosphoCACT UBI SLMB
stoichiometric coefficient: 1.0

DIF
type: Complex
component stoichiometry: DIF s153 sa62 STOICHIOMETRY ID COMPLEX
component: DIF
feature: COMPLEX PARTICIPANT BINDING FEATURE s153 sa62 68
cellular location: cytosol

PARTICIPANT STOICHIOMETRY re29 194
type: Stoichiometry
physical entity: DL dimer phosphoCACT SLMB
stoichiometric coefficient: 1.0

DIF
type: Complex
component stoichiometry: DIF s155 sa98 STOICHIOMETRY ID COMPLEX
component: DIF
feature: COMPLEX PARTICIPANT BINDING FEATURE s155 sa98 90
cellular location: cytosol

TL s146 sa51 STOICHIOMETRY ID COMPLEX
type: Stoichiometry
physical entity: TL
stoichiometric coefficient: 2.0

DL
type: Complex
component stoichiometry: DL s112 sa76 STOICHIOMETRY ID COMPLEX
component: DL
feature: COMPLEX PARTICIPANT BINDING FEATURE s112 sa76 134
cellular location: cytosol

DL
type: Complex
component stoichiometry: DL s103 sa72 STOICHIOMETRY ID COMPLEX
component: DL
feature: COMPLEX PARTICIPANT BINDING FEATURE s103 sa72 146
cellular location: cytosol

DIF
type: Complex
component stoichiometry: DIF s168 sa107 STOICHIOMETRY ID COMPLEX
component: DIF
cellular location: cytosol

DL
type: Complex
component stoichiometry: DL s118 sa81 STOICHIOMETRY ID COMPLEX
component: DL
feature: COMPLEX PARTICIPANT BINDING FEATURE s118 sa81 92
cellular location: cytosol

DL
type: Complex
component stoichiometry: DL s128 sa89 STOICHIOMETRY ID COMPLEX
component: DL
cellular location: nucleus

DL
type: Complex
component stoichiometry: DL s123 sa87 STOICHIOMETRY ID COMPLEX
component: DL
cellular location: cytosol

DL
type: Complex
component stoichiometry: DL s94 sa64 STOICHIOMETRY ID COMPLEX
component: DL
feature: COMPLEX PARTICIPANT BINDING FEATURE s94 sa64 132
cellular location: cytosol

DL
type: Complex
component stoichiometry: DL s91 sa61 STOICHIOMETRY ID COMPLEX
component: DL
feature: COMPLEX PARTICIPANT BINDING FEATURE s91 sa61 78
cellular location: cytosol

uniprot Q05652 14
type: UnificationXref
id: Q05652
db: uniprot