#! /usr/local/bin/perl ##### ##### ## ## This script is to map the id in your gene list to PANTHER classificaiton. ## ## Inputs: ## -i The PANTHER classification file for the genome of your interest ## -g Your gene list (tab-deliminated simple text file with the UniProt ID ## in the first column) ## ##### ##### # get command-line arguments use Getopt::Std; getopts('o:i:g:e:vVh') || &usage(); &usage() if ($opt_h); # -h for help $outDir = $opt_o if ($opt_o); # -o for (o)utput directory $inFile = $opt_i if ($opt_i); # -i for the input pthr mapping file $geneList = $opt_g if ($opt_g); # -g for the gene list $errFile = $opt_e if ($opt_e); # -e for (e)rror file (redirect STDERR) $verbose = 1 if ($opt_v); # -v for (v)erbose (debug info to STDERR) $verbose = 2 if ($opt_V); # -V for (V)ery verbose (debug info STDERR) my %pthr; open (FH, $inFile); while (my $line=){ chomp $line; my ($id, $pthr)=split(/\t/, $line); $pthr{$id}=$pthr; } close (FH); my $count1; my $count2; open (GL, $geneList); while (my $line=){ $line=~s/\r//g; chomp $line; my ($id, @rest)=split(/\t/, $line); if (defined $pthr{$id}){ $count1++; my $pthr=$pthr{$id}; my $foo = join("\t", @rest); print "$id\t$pthr\t$foo\n"; }else{ $count2++; print "$id\tNOHIT\t$foo\n"; } } close (GL); print STDERR "$count1 IDs can be mapped.\n"; print STDERR "$count2 IDs are not mapped.\n";